Coding

Part:BBa_K2997001:Design

Designed by: Ting-Han Sung   Group: iGEM19_NCKU_Tainan   (2019-10-12)


Bacteriocin full length (CBM-B)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 396
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 396
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 396
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 396
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the original designs for inhibiting clostridium strains and thus lowering p-cresol production in gut, bacteriocin is fused with a secretion tag, yebF. YebF is a secretory protein with unknown function in e.coli[3]. We first amplify the yebF fragments from the genome of e.coli MG1655 and bacteriocin


Source

Luckily, igem ncku 2019 was kindly supported by one of our instructor PI, who is currently devoting himself in the field of developing novel therapeutic approach for c.diff infection. And he kindly provide us a plasmid cloned with a bacteriocin gene(CBM-B) from CBM, which has been proven to have the bactericidal ability to certain strain of clostridium, including c.diff[2].


References